The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
Blog Article
HPLC works pursuing The essential theory of slim layer chromatography or column chromatography, wherever it's a stationary period in addition to a mobile phase. The mobile period flows in the stationary section and carries the parts from the mixture with it.
The sample injector is utilized to inject the sample in the HPLC system. To attain correct elution, the sample is Typically dissolved in an appropriate solvent that matches the cell section.
전자를 '고정상', 후자를 '이동상'이라 부르며 크로마토그래피에서는 분석자는 고정상과 이동상의 조합에 의해 분석물의 분리를 제어할 수 있게 됩니다.따라서 분석물, 고정상, 이동상, 세 가지 특성의 이해가 크로마트그래피에서 매우 중요합니다.
The cell stage could be the solvent combination that consistently flows throughout the HPLC system, carrying the sample through the column. It performs a vital purpose in separating the analytes:
. Solvent triangle for optimizing a reversed-stage HPLC separation. The 3 blue circles clearly show cell phases consisting of an organic solvent and h2o.
テキストはクリエイティブ・コモンズ 表示-継承ライセンスのもとで利用できます。追加の条件が適用される場合があります。詳細については利用規約を参照してください。
Preserve a logbook: Document your observations, which includes peak shapes, retention times, and any changes manufactured to the tactic. This will let you establish traits and troubleshoot challenges more effectively.
Establishing an optimized HPLC technique includes strategically changing several parameters to achieve the absolute best separation on your precise analytes. Critical parameters for optimization contain:
The data acquisition system controls the HPLC instrument and collects the sign through the detector. This data is displayed as being a chromatogram, working of hplc system a graph showing peaks corresponding to the divided analytes.
Ordinary-section: Separates determined by polarity. Analytes with higher polarity interact extra Together with the polar stationary section and elute later on.
takes advantage of an autosampler to inject samples. Instead of using a syringe to push the sample in to the sample loop, the syringe attracts sample into the sample loop.
During this section we consider the fundamental plumbing needed to transfer the cell section throughout the column and to inject the sample into your get more info mobile section.
Just after loading the sample, the injector is turned into the inject place, which redirects the mobile phase in the sample loop and on to the column.
Privateness Overview This Web site utilizes cookies to make sure that we will supply you with the most effective person expertise probable. Cookie information and facts is stored in the browser and performs functions for instance recognising you when you come to our website and serving to our team to know which sections of the website you discover most exciting and useful.